|Description||RAT IgM whole molecule|
|Physical State||Liquid (sterile filtered)|
|Buffer||0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0|
|Species of Origin||Rat|
|Preservative||0.1% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Rat IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Rat IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Rat IgM ( µ chain specific). No reaction was observed against anti-Rat IgG F(c). Some light chain cross-reactivity will occur with anti-Rat IgG.|
|Storage Condition||Store vial at 4° C prior to opening. Rat IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
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