Active AHCY, human recombinant protein
SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosyl Homocysteinase
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P23526 |
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Concentration | 5 |
Calculated MW | 52.1 kDa (1-432 aa + N-terminal polyhistidine tag). In the native form the protein exists in the tetrameric form and this form is required for activity. |
Gene ID | 191 |
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Gene Symbol | AHCY |
Other Names | SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosyl Homocysteinase |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | BioVision’s Active AHCY has been tested for its activity using Adenosyl Homocysteinase (AHCY) Activity Fluorometric Assay Kit (K807-100). It has a specific activity of >350 mU/mg. |
Target/Specificity | AHCY/SAHH |
Format | Liquid |
Storage | -80°C; 5 mg/ml in 50 mM potassium phosphate, 1 mM EDTA, pH 7.2 containing 20% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
AHCY (EC 3.3.1.1) is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AHCY controls the intracellular S-adenosylhomocysteine (SAH) concentration that is crucial for transmethylation reactions. AHCY deficiency causes hypermethioninemia. The recombinant human AHCY produced in E.Coli is a single, non-glycosylated polypeptide chain and is purified by proprietary chromatographic techniques.
References
Coulter-Karis D.E.,et al.Ann. Hum. Genet. 53:169-175(1989).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 414:865-871(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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