Active Caspase-1, rat recombinant protein
Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P43527 |
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Calculated MW | ~ 34.6 KDa |
Gene Symbol | Casp1 |
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Other Names | Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45. |
Gene Source | Rat |
Source | E. coli |
Assay&Purity | N/A; |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | >3000 units/mg |
Target/Specificity | Caspase-1 |
Application Notes | Reconstitute to 1 unit per µl in PBS containing 15% glycerol. |
Format | Semi-Dry |
Storage | -80°C; Semi-Dry |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant rat caspase-1 was expressed in E. coli as a single polypeptide of 284 amino acids with molecular weight of 34.6. The caspase-1 is purified and activated by proprietary techniques.
References
Keane K.M.,et al.Cytokine 7:105-110(1995).
Flaws J.A.,et al.Endocrinology 136:5042-5053(1995).
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