Active Human Calpain 1 recombinant protein
Calpain
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P07384 |
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Calculated MW | 112.0 kDa |
Gene ID | 823 |
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Gene Symbol | CAPN1 |
Other Names | Calcium-activated neutral proteinase 2, Short name=CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, Short name=M-calpain |
Gene Source | Human |
Source | Human Erythrocyte |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | No |
Target/Specificity | Calpain 1 |
Format | Liquid |
Storage | -70°C; In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ca²ᶧ-dependent cysteine proteinase with low Ca²ᶧrequirement (half maximal activation = 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin. Involves in apoptosis activation.
References
Aoki K.,et al.FEBS Lett. 205:313-317(1986).
Sorimachi H.,et al.Biol. Chem. Hoppe-Seyler 371:171-176(1990).
Kim J.W.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
Melloni E.,et al.Biochem. Biophys. Res. Commun. 229:193-197(1996).
Michetti M.,et al.FEBS Lett. 392:11-15(1996).
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