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HLA-B Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HLA-B Antibody (N-term) AP8616a
    All lanes : Anti-HLA-B Antibody (N-term) at 1:1000-2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Ramos whole cell lysate Lane 5: A375 whole cell lysate Lane 6: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - HLA-B Antibody (N-term) AP8616a
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using AP8616a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - HLA-B Antibody (N-term) AP8616a
    Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, Cat#AP8616a) compared to an isotype control of rabbit IgG (blue). AP8616a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, FC, WB, E
Primary Accession P03989
Other Accession P30490, P30488, P30487, P30486, P30485, P30483, P30481, P30479, Q04826, P18463, P30464, P30461
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 62-90 aa
Additional Information
Other Names HLA class I histocompatibility antigen, B-27 alpha chain, MHC class I antigen B*27, HLA-B, HLAB
Target/Specificity This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
Dilution WB~~1:1000-2000
IHC-P-Leica~~1:500
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

HLA-B is involved in the presentation of foreign antigens to the immune system.

References

Higgins,C.M., et.al., Ann. Rheum. Dis. 51 (7), 855-862 (1992)
Guild,B.C.et.al., J. Biol. Chem. 259 (14), 9235-9240 (1984)
Orr,H.T., et.alt., Biochemistry 18 (25), 5711-5720 (1979)

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$ 365.00
$ 140.00
Cat# AP8616a
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