Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Phospho-MeCP2(S423) Antibody   

Phospho-MeCP2(S423) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P-Leica - Phospho-MeCP2(S423) Antibody AP3693a
    Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP3693a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • WB - Phospho-MeCP2(S423) Antibody AP3693a
    Western blot analysis of lysates from C6 cell line, untreated or treated with calf intestinal alkaline phosphatase(CIP), using Phospho-MeCP2(S423) Antibody(upper) or Beta-actin (lower).
  • WB - Phospho-MeCP2(S423) Antibody AP3693a
    Western blot analysis of lysates from Jurkat cell line, untreated or treated with calf intestinal alkaline phosphatase(CIP), using Phospho-MeCP2(S423) Antibody(upper) or Beta-actin (lower).
  • WB - Phospho-MeCP2(S423) Antibody AP3693a
    Western blot analysis of lysates from Neuro-2a cell line, untreated or treated with calf intestinal alkaline phosphatase(CIP), using Phospho-MeCP2(S423) Antibody(upper) or Beta-actin (lower).
  • SPECIFICATION
  • CITATIONS: 5
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, WB, E
Primary Accession P51608
Other Accession Q9Z2D6, Q95LG8
Reactivity Human, Rat
Predicted Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52441 Da
Additional Information
Gene ID 4204
Other Names Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
Target/Specificity This MeCP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S423 of human MeCP2.
Dilution IHC-P-Leica~~1:500
WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-MeCP2(S423) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MECP2
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q9Z2D6}. Note=Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.
Tissue Location Present in all adult somatic tissues tested.
Research Areas
Citations ( 0 )

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

References

Mnatzakanian, G.N., et al., Nat. Genet. 36(4):339-341 (2004). Laccone, F., et al., Hum. Mutat. 23(3):234-244 (2004). Suzuki, M., et al., Oncogene 22(54):8688-8698 (2003). Balmer, D., et al., J. Mol. Med. 81(1):61-68 (2003). Hagberg, B., et al., Eur. J. Paediatr. Neurol. 7(6):417-421 (2003).

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

$ 325.00
$ 109.00
Cat# AP3693a
Size:
Quantity:
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"