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Vimentin Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- SPECIFICATION
- CITATIONS: 8
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, FC, WB, IF, E |
|---|---|
| Primary Accession | P08670 |
| Other Accession | P31000, P02543, P20152, Q4R4X4, P48670, P09654, P48616, P24790 |
| Reactivity | Human |
| Predicted | Xenopus, Bovine, Chicken, Hamster, Monkey, Mouse, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 53652 Da |
| Antigen Region | 430-457 aa |
| Gene ID | 7431 |
|---|---|
| Other Names | Vimentin, VIM |
| Target/Specificity | This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin. |
| Dilution | IF~~1:100 WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Vimentin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | VIM |
|---|---|
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152} |
| Tissue Location | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
Provided below are standard protocols that you may find useful for product applications.
Background
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
References
References for protein:
1.Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008)
2.Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008)
3.Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704]
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