- CITATIONS: 13
|Application ||WB, FC, E|
|Other Accession||O02703, Q07814|
|Calculated MW||21184 Da|
|Antigen Region||41-76 aa|
|Other Names||Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BAX, BCL2L4|
|Target/Specificity||This Bax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-76 amino acids from human Bax.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bax Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.|
|Cellular Location||Isoform Alpha: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane Isoform Gamma: Cytoplasm.|
|Tissue Location||Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.|
Provided below are standard protocols that you may find useful for product applications.
Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003). Roucou, X., et al., J. Biol. Chem. 278(42):40877-40881 (2003). Cao, X., et al., Blood 102(7):2605-2614 (2003). McJilton, M.A., et al., Oncogene 22(39):7958-7968 (2003). Bidere, N., et al., J. Biol. Chem. 278(33):31401-31411 (2003).
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