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DNAJB11 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9UBS4 |
| Other Accession | Q6TUG0, Q99KV1, Q3ZBA6, NP_057390.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 40514 Da |
| Antigen Region | 63-92 aa |
| Gene ID | 51726 |
|---|---|
| Other Names | DnaJ homolog subfamily B member 11, APOBEC1-binding protein 2, ABBP-2, DnaJ protein homolog 9, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, Endoplasmic reticulum DNA J domain-containing protein 3, ER-resident protein ERdj3, ERdj3, ERj3p, HEDJ, Human DnaJ protein 9, hDj-9, PWP1-interacting protein 4, DNAJB11, EDJ, ERJ3, HDJ9 |
| Target/Specificity | This DNAJB11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human DNAJB11. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DNAJB11 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DNAJB11 |
|---|---|
| Synonyms | EDJ, ERJ3, HDJ9 |
| Function | As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins (PubMed:10827079, PubMed:15525676, PubMed:29706351). Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed (PubMed:15525676). May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity (PubMed:10827079). It is necessary for maturation and correct trafficking of PKD1 (PubMed:29706351). |
| Cellular Location | Endoplasmic reticulum lumen Note=Associated with the ER membrane in a C-terminally epitope-tagged construct |
| Tissue Location | Widely expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DNAJB11 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a C-terminal cysteine-rich region (Ohtsuka and Hata, 2000 [PubMed 11147971]).
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wen, K.W., et al. Oncogene 29(24):3532-3544(2010)
Bernal-Bayard, J., et al. J. Biol. Chem. 285(21):16360-16368(2010)
Vembar, S.S., et al. J. Biol. Chem. 284(47):32462-32471(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
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