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PROC Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PROC Antibody AP1017a
    Western blot analysis of anti-Protein-C tag Pab (Cat. #AP1017a)in Protein-C-tagged recombinant protein bacterial lysate.
    detail
  • WB - PROC Antibody AP1017a
    Anti-PROC Antibody at 1:1000 dilution + H.plasma whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P04070
Reactivity Human
Predicted Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52071 Da
Additional Information
Gene ID 5624
Other Names Vitamin K-dependent protein C, Anticoagulant protein C, Autoprothrombin IIA, Blood coagulation factor XIV, Vitamin K-dependent protein C light chain, Vitamin K-dependent protein C heavy chain, Activation peptide, PROC
Target/Specificity KLH conjugated synthetic peptide encoding the Protein-C epitope tag (CEDQVDPRLIDGK) was used as antigen.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPROC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PROC
Function Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
Cellular Location Secreted. Golgi apparatus Endoplasmic reticulum
Tissue Location Plasma; synthesized in the liver.
Research Areas
Citations (0)
citation

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Background

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The epitope tag composed of a 12 residue peptide, EDQVDPRLIDGK , is derived from Protein-C, a vitamin K-dependent serine protease that regulates blood coagluation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Abgent's anti-Protein-C polyclonal antibody provides a simple solution to detect the expression of a Protein-C epitope-tagged protein in cells.

References

Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S. Comparison of affinity tags for protein purification. Protein Expr Purif. 2005 May;41(1):98-105.

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$ 365.00
$ 140.00
Cat# AP1017a
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