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USP22 Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

     
  • WB - USP22 Antibody (C-term) AM2230b
    USP22 Antibody (C-term)(Cat. #AM2230b) western blot analysis in Daudi cell line lysates (35μg/lane).This demonstrates the USP22 antibody detected the USP22 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UPT9
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 1154CT13.1.3.1
Calculated MW 59961 Da
Additional Information
Gene ID 23326
Other Names Ubiquitin carboxyl-terminal hydrolase 22, Deubiquitinating enzyme 22, Ubiquitin thioesterase 22, Ubiquitin-specific-processing protease 22, USP22, KIAA1063, USP3L
Target/Specificity Purified His-tagged USP22 protein was used to produced this monoclonal antibody.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP22 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP22
Synonyms KIAA1063, USP3L
Function Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q5DU02}.
Tissue Location Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver
Research Areas
Citations (0)
citation

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Background

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.

References

Kikuno R., et al. DNA Res. 6:197-205(1999).
Bechtel S., et al. BMC Genomics 8:399-399(2007).
Lee H.-J., et al. Gene Expr. Patterns 6:277-284(2006).
Zhao Y., et al. Mol. Cell 29:92-101(2008).
Zhang X.-Y., et al. Mol. Cell 29:102-111(2008).

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$ 182.50
$ 70.00
Cat# AM2230b
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