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NME1 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • WB - NME1 Antibody AM2209b
    NME1 Antibody(Cat. #AM2209b) western blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the NME1 antibody detected the NME1 protein (arrow).
    detail
  • IHC-P - NME1 Antibody AM2209b
    Immunohistochemical analysis of paraffin-embedded H. heart section using NME1 Antibody(Cat#AM2209B). AM2209B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • IHC-P - NME1 Antibody AM2209b
    Immunohistochemical analysis of paraffin-embedded H. spleen section using NME1 Antibody(Cat#AM2209B). AM2209B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • SPECIFICATION
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P15531
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone/Animal Names 1172CT2.4.1.1
Calculated MW 17149 Da
Additional Information
Gene ID 4830
Other Names Nucleoside diphosphate kinase A, NDK A, NDP kinase A, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NME1, NDPKA, NM23
Target/Specificity Purified His-tagged NME1 protein was used to produced this monoclonal antibody.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNME1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NME1
Synonyms NDPKA, NM23
Function Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA- mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Cellular Location Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA
Tissue Location Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation.
Research Areas
Citations ( 0 )

Background

Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.

References

Rosengard A.M., et al. Nature 342:177-180(1989).
Gilles A.-M., et al. J. Biol. Chem. 266:8784-8789(1991).
Wang L., et al. Cancer Res. 53:717-720(1993).
Dooley S., et al. Hum. Genet. 93:63-66(1994).
Ni X., et al. J. Hum. Genet. 48:96-100(2003).

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$ 365.00
$ 140.00
Cat# AM2209b
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