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ACTB / Beta Actin Antibody (clone 1E7)

Mouse Monoclonal Antibody

     
  • WB - ACTB / Beta Actin Antibody (clone 1E7) ALS14305
    Western blot of ACTB expression in Jurkat.
    detail
  • WB - ACTB / Beta Actin Antibody (clone 1E7) ALS14305
    Western blot of ACTB expression in NIH/3T3.
    detail
  • IHC - ACTB / Beta Actin Antibody (clone 1E7) ALS14305
    Anti-Beta Actin antibody IHC of human prostate, stroma.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P60709
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Clone Names 1E7
Calculated MW 42kDa
Dilution IHC-P (5 µg/ml), WB (1:500-1:1000),
Additional Information
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity Human Beta Actin
Reconstitution & Storage Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
PrecautionsACTB / Beta Actin Antibody (clone 1E7) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTB
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Research Areas
Citations (0)
citation

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Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

References

Ponte P.,et al.Nucleic Acids Res. 12:1687-1696(1984).
Nakajima-Iijima S.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985).
Ohmori H.,et al.J. Cell Biol. 116:933-941(1992).
Hillier L.W.,et al.Nature 424:157-164(2003).
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).

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$ 533.50
Cat# ALS14305
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