Anti-Human Adiponectin Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Preparation | Produced in Balb/c mice using highly pure recombinant Human Adiponectin as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography. |
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WesternBlot | To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions. |
Sandwich | In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μg/ml. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Anti-Human Adiponectin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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