Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.ELITE™ Modification-state-specific Antibody services
Phospho-specific antibodies are critical tools used for research of signaling pathways and regulatory networks in various cellular processes. Abcepta has vast experience in the development of custom and off the shelf antibodies against specific phosphorylated residues in different protein targets.
Additionally, Abcepta can also develop polyclonal /monoclonal antibodies against methylated and acetylated residues, valuable tools in epigenetics research.
Our project managers will work with you from antigen design to validation.
Our ELITE™ Modification-state-specific antibody offering includes
the following:
| Services | Days |
|
Antigen design and preparation: Modified peptide synthesis Peptide conjugation to KLH |
7 14 |
|
Control non modified peptide synthesis Peptide conjugation to KLH |
7 14 |
| Animal immunization: rabbits for polyclonal and mouse for monoclonal | 1 |
| Test bleed ELISA screening for phosphopeptide, non-phosphopeptide | 35-49 |
|
Hybridoma fusion and screening for phosphopeptide, non-phosphopeptide clones, clones amplification and storage |
50 |
| Ascites production | 85 |
| Two step phosho-specific purification by peptide affinity | 99 |
| Validation test by WB (limited guarantee available) | 3 |
| Validation test by IF (limited guarantee available) | 3 |
| Validation by other tests (limited guarantee available) | 3-10 |
| Total: | ~120-140 |
Deliverables:
- 2 x 1 ml pre-immune bleeds
- 2 x 1 ml 1st post-immune bleeds
- 10 mg of peptide, 2-10 mg of Ig fraction (protein A and peptide affinity purified)
Abcepta also provides blocking peptides for phospho-specific antibodies.
These peptides are used to confirm that signal specificity is due to the antibody's interaction with its antigen, as opposed to non-specific binding.
Email:
USA: sales@abcepta.com
Phone:
USA: 888.735.7227 (toll free) or 858.875.1900
Fax:
USA: 858-622-0609
